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protein 4 fabp4 inhibitor  (MedChemExpress)


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    MedChemExpress protein 4 fabp4 inhibitor
    Proteomic profile revealing the change during liver transplantation. A Heatmap of the differentially expressed proteins. Red rectangles mean that proteins are upregulated, and green ones mean that they are downregulated. B Principal component analysis of the duplicate samples, in which the degree of aggregation among samples represents statistical consistency. C Volcano plot of the differentially expressed proteins between NC SHAM and HF SHAM groups. Gray dots represent genes that are not differentially expressed; red dots and blue dots represent genes that are upregulated and downregulated significantly. D Volcano plot of the differentially expressed proteins between NC LT and HF LT groups. E Venn diagram demonstrating shared and discrete proteins in each of these four groups. F Expression patterns of these differentially expressed proteins based on membership and expression. G Protein–protein interaction network of differentially expressed proteins with <t>FABP4</t>
    Protein 4 Fabp4 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein 4 fabp4 inhibitor/product/MedChemExpress
    Average 94 stars, based on 31 article reviews
    protein 4 fabp4 inhibitor - by Bioz Stars, 2026-02
    94/100 stars

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    1) Product Images from "Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation"

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    Journal: Cellular and Molecular Life Sciences

    doi: 10.1007/s00018-023-05110-1

    Proteomic profile revealing the change during liver transplantation. A Heatmap of the differentially expressed proteins. Red rectangles mean that proteins are upregulated, and green ones mean that they are downregulated. B Principal component analysis of the duplicate samples, in which the degree of aggregation among samples represents statistical consistency. C Volcano plot of the differentially expressed proteins between NC SHAM and HF SHAM groups. Gray dots represent genes that are not differentially expressed; red dots and blue dots represent genes that are upregulated and downregulated significantly. D Volcano plot of the differentially expressed proteins between NC LT and HF LT groups. E Venn diagram demonstrating shared and discrete proteins in each of these four groups. F Expression patterns of these differentially expressed proteins based on membership and expression. G Protein–protein interaction network of differentially expressed proteins with FABP4
    Figure Legend Snippet: Proteomic profile revealing the change during liver transplantation. A Heatmap of the differentially expressed proteins. Red rectangles mean that proteins are upregulated, and green ones mean that they are downregulated. B Principal component analysis of the duplicate samples, in which the degree of aggregation among samples represents statistical consistency. C Volcano plot of the differentially expressed proteins between NC SHAM and HF SHAM groups. Gray dots represent genes that are not differentially expressed; red dots and blue dots represent genes that are upregulated and downregulated significantly. D Volcano plot of the differentially expressed proteins between NC LT and HF LT groups. E Venn diagram demonstrating shared and discrete proteins in each of these four groups. F Expression patterns of these differentially expressed proteins based on membership and expression. G Protein–protein interaction network of differentially expressed proteins with FABP4

    Techniques Used: Transplantation Assay, Expressing

    Relationship with FABP4 expression and liver transplantation recipients’ overall survival. A FABP4 is a high expression of donors’ liver tissue microarray IHC staining. B FABP4 low expression of IHC staining. C Comparison of overall survival rate between FABP4 high- and low-expression groups. D Comparison of the incidence of early allograft dysfunction. E Comparison of the risk of liver steatosis
    Figure Legend Snippet: Relationship with FABP4 expression and liver transplantation recipients’ overall survival. A FABP4 is a high expression of donors’ liver tissue microarray IHC staining. B FABP4 low expression of IHC staining. C Comparison of overall survival rate between FABP4 high- and low-expression groups. D Comparison of the incidence of early allograft dysfunction. E Comparison of the risk of liver steatosis

    Techniques Used: Expressing, Transplantation Assay, Microarray, Immunohistochemistry, Comparison

    Influence of FABP4 inhibitor on mouse high fatty liver transplantation model. A H&E staining of mouse liver transplantation model after using FABP4 inhibitor. B TUNEL staining. C – F Liver function detection of ALT, AST, LDH, and TB. G – I Oxidative stress injury assay of GSH, MDA, and SOD. J – N Relative expression of FABP4, Bax, cleaved Caspase-3, and cleaved PARP. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001
    Figure Legend Snippet: Influence of FABP4 inhibitor on mouse high fatty liver transplantation model. A H&E staining of mouse liver transplantation model after using FABP4 inhibitor. B TUNEL staining. C – F Liver function detection of ALT, AST, LDH, and TB. G – I Oxidative stress injury assay of GSH, MDA, and SOD. J – N Relative expression of FABP4, Bax, cleaved Caspase-3, and cleaved PARP. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Techniques Used: Transplantation Assay, Staining, TUNEL Assay, Expressing

    Transcriptomic profiles revealing the influence of FABP4 inhibitor. A Violin diagram of expression patterns of all genes. B Principal component analysis of the duplicate samples. C Heatmap of the differentially expressed genes. D Volcano plot of the differentially expressed genes between HF LT and HF BMS groups. E Gene ontology pathway enrichment for differentially expressed proteins. The circle sizes represent the number of genes enriched in pathways, and the circle’s color means significance. F KEGG pathway enrichment for differentially expressed proteins
    Figure Legend Snippet: Transcriptomic profiles revealing the influence of FABP4 inhibitor. A Violin diagram of expression patterns of all genes. B Principal component analysis of the duplicate samples. C Heatmap of the differentially expressed genes. D Volcano plot of the differentially expressed genes between HF LT and HF BMS groups. E Gene ontology pathway enrichment for differentially expressed proteins. The circle sizes represent the number of genes enriched in pathways, and the circle’s color means significance. F KEGG pathway enrichment for differentially expressed proteins

    Techniques Used: Expressing

    Metabolomic profiles revealing the influence of FABP4 inhibitor. A Total ion chromatogram of spectral overlap comparison, with response intensity and retention time overlapping. B Principal component analysis of the duplicate samples. C Volcano plot of the differentially expressed metabolites between HF LT and HF BMS groups. D Heatmap of the differentially expressed metabolites. E Correlation analysis between metabolites and visualized in the form of correlation heatmaps. F Revealing the co-regulatory relationships between various metabolites by chord diagrams
    Figure Legend Snippet: Metabolomic profiles revealing the influence of FABP4 inhibitor. A Total ion chromatogram of spectral overlap comparison, with response intensity and retention time overlapping. B Principal component analysis of the duplicate samples. C Volcano plot of the differentially expressed metabolites between HF LT and HF BMS groups. D Heatmap of the differentially expressed metabolites. E Correlation analysis between metabolites and visualized in the form of correlation heatmaps. F Revealing the co-regulatory relationships between various metabolites by chord diagrams

    Techniques Used: Comparison

    Influence of FABP4 inhibitor on cAMP signaling pathway. A – E Relative expression of HHIP, ADRB2, RAC2, and PKA. F , G The content of Prostaglandin i2 and N-oleoylethanolamine validated by ELISA. H – K The mRNA expression of Hhip, Adrb2, Rac2, and Adcy7 of HF LT and HF BMS groups. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001
    Figure Legend Snippet: Influence of FABP4 inhibitor on cAMP signaling pathway. A – E Relative expression of HHIP, ADRB2, RAC2, and PKA. F , G The content of Prostaglandin i2 and N-oleoylethanolamine validated by ELISA. H – K The mRNA expression of Hhip, Adrb2, Rac2, and Adcy7 of HF LT and HF BMS groups. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    Influence of FABP4 inhibitor on high fatty liver mitochondrion. A – C Transmission electron microscopy image of mouse liver in each group. A The mitochondrial structure was clear, and no autophagosomes were presented in the HF SHAM group. B Autophagosomes and autolysosomes were found in the HF LT group with mitochondrial swelling. C The mitochondrial structure of the HF BMS group was clear, and damaged parts of mitochondria were lytic. D – F Multiplex immunofluorescence staining of rhodamine reagent for detecting mitochondrial membrane potential. Green fluorescence represents mitochondrial membranous potential, red fluorescence represents apoptotic hepatocytes, and blue fluorescence represents nuclear staining. G – I Relative expression of DRP1 and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. AP, autophagosomes; ASS, autolysosome; LD, lipid droplets; M, mitochondria; RER, rough endoplasmic reticulum
    Figure Legend Snippet: Influence of FABP4 inhibitor on high fatty liver mitochondrion. A – C Transmission electron microscopy image of mouse liver in each group. A The mitochondrial structure was clear, and no autophagosomes were presented in the HF SHAM group. B Autophagosomes and autolysosomes were found in the HF LT group with mitochondrial swelling. C The mitochondrial structure of the HF BMS group was clear, and damaged parts of mitochondria were lytic. D – F Multiplex immunofluorescence staining of rhodamine reagent for detecting mitochondrial membrane potential. Green fluorescence represents mitochondrial membranous potential, red fluorescence represents apoptotic hepatocytes, and blue fluorescence represents nuclear staining. G – I Relative expression of DRP1 and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. AP, autophagosomes; ASS, autolysosome; LD, lipid droplets; M, mitochondria; RER, rough endoplasmic reticulum

    Techniques Used: Transmission Assay, Electron Microscopy, Multiplex Assay, Immunofluorescence, Staining, Membrane, Fluorescence, Expressing

    Influence of FABP4 siRNA on in vitro hypoxia / reoxygenation model. A – D Flow cytometry was performed to detect the hypoxic injury-induced apoptosis of AML12 cells. E – H Liver function detection of ALT, AST, LDH, and TB using the supernatant of each group. I – P Relative expression of FABP4, PKA, RAC2, HHIP, ADRB, DRP1, and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001
    Figure Legend Snippet: Influence of FABP4 siRNA on in vitro hypoxia / reoxygenation model. A – D Flow cytometry was performed to detect the hypoxic injury-induced apoptosis of AML12 cells. E – H Liver function detection of ALT, AST, LDH, and TB using the supernatant of each group. I – P Relative expression of FABP4, PKA, RAC2, HHIP, ADRB, DRP1, and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Techniques Used: In Vitro, Flow Cytometry, Expressing



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    Proteomic profile revealing the change during liver transplantation. A Heatmap of the differentially expressed proteins. Red rectangles mean that proteins are upregulated, and green ones mean that they are downregulated. B Principal component analysis of the duplicate samples, in which the degree of aggregation among samples represents statistical consistency. C Volcano plot of the differentially expressed proteins between NC SHAM and HF SHAM groups. Gray dots represent genes that are not differentially expressed; red dots and blue dots represent genes that are upregulated and downregulated significantly. D Volcano plot of the differentially expressed proteins between NC LT and HF LT groups. E Venn diagram demonstrating shared and discrete proteins in each of these four groups. F Expression patterns of these differentially expressed proteins based on membership and expression. G Protein–protein interaction network of differentially expressed proteins with <t>FABP4</t>
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    Determination of adipocyte-specific markers and AGMO activity during 3T3-L1 adipocyte differentiation. A: Oil Red O staining of differentiated (day 11, upper panel) and undifferentiated (day 0, lower panel) 3T3-L1 cells. B: Gene expression of late adipocyte markers adiponectin ( Adipoq ), leptin ( Lep ), peroxisome proliferator-activated receptor gamma ( Pparg ), and fatty acid-binding protein 4 ( <t>Fabp4</t> ) was analyzed by RT-qPCR using TaqMan technology. Open bars and open circles represent undifferentiated cells at day 0 prior to differentiation; gray bars, full circles correspond to mature adipocytes at day 11 after exposure of 3T3-L1 cells to the adipocyte differentiation medium 1 and 2 (n = 3). C: AGMO enzymatic activity in cell pellets of 3T3-L1 exposed to the basal medium at day 0 (open bars and open circles) and cells supplemented with the differentiation medium 1 and 2 at day 11 (gray bars and full circles) of adipogenesis (n = 3). D: Agmo gene expression of cells at day 0 (open bars and open circles) and day 11 (gray bars and full circles) of adipocyte differentiation. E: Time course of AGMO activity during differentiation of 3T3-L1 cells exposed to the standard hormonal differentiation cocktail (diff. med. control) or a hormonal induction medium devoid of either IBMX, RGZ, DEX, or IBMX/RGZ and DEX/RGZ in combination (n = 5). F: Lipid droplet quantification by Bodipy and Hoechst staining using the CellProfiler™ analysis software of 3T3-L1 cells at day 0 and day 11 incubated with the complete differentiation medium (control) or differentiation medium with omitted IBMX, RGZ, DEX, or IBMX/RGZ and DEX/RGZ (three areas per well; n = 4). Mean ± SEM except for the boxplots in F, which show the median ± interquartile range (IQR). Whiskers range from minimum to maximum. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.
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    Proteomic profile revealing the change during liver transplantation. A Heatmap of the differentially expressed proteins. Red rectangles mean that proteins are upregulated, and green ones mean that they are downregulated. B Principal component analysis of the duplicate samples, in which the degree of aggregation among samples represents statistical consistency. C Volcano plot of the differentially expressed proteins between NC SHAM and HF SHAM groups. Gray dots represent genes that are not differentially expressed; red dots and blue dots represent genes that are upregulated and downregulated significantly. D Volcano plot of the differentially expressed proteins between NC LT and HF LT groups. E Venn diagram demonstrating shared and discrete proteins in each of these four groups. F Expression patterns of these differentially expressed proteins based on membership and expression. G Protein–protein interaction network of differentially expressed proteins with FABP4

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Proteomic profile revealing the change during liver transplantation. A Heatmap of the differentially expressed proteins. Red rectangles mean that proteins are upregulated, and green ones mean that they are downregulated. B Principal component analysis of the duplicate samples, in which the degree of aggregation among samples represents statistical consistency. C Volcano plot of the differentially expressed proteins between NC SHAM and HF SHAM groups. Gray dots represent genes that are not differentially expressed; red dots and blue dots represent genes that are upregulated and downregulated significantly. D Volcano plot of the differentially expressed proteins between NC LT and HF LT groups. E Venn diagram demonstrating shared and discrete proteins in each of these four groups. F Expression patterns of these differentially expressed proteins based on membership and expression. G Protein–protein interaction network of differentially expressed proteins with FABP4

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: Transplantation Assay, Expressing

    Relationship with FABP4 expression and liver transplantation recipients’ overall survival. A FABP4 is a high expression of donors’ liver tissue microarray IHC staining. B FABP4 low expression of IHC staining. C Comparison of overall survival rate between FABP4 high- and low-expression groups. D Comparison of the incidence of early allograft dysfunction. E Comparison of the risk of liver steatosis

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Relationship with FABP4 expression and liver transplantation recipients’ overall survival. A FABP4 is a high expression of donors’ liver tissue microarray IHC staining. B FABP4 low expression of IHC staining. C Comparison of overall survival rate between FABP4 high- and low-expression groups. D Comparison of the incidence of early allograft dysfunction. E Comparison of the risk of liver steatosis

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: Expressing, Transplantation Assay, Microarray, Immunohistochemistry, Comparison

    Influence of FABP4 inhibitor on mouse high fatty liver transplantation model. A H&E staining of mouse liver transplantation model after using FABP4 inhibitor. B TUNEL staining. C – F Liver function detection of ALT, AST, LDH, and TB. G – I Oxidative stress injury assay of GSH, MDA, and SOD. J – N Relative expression of FABP4, Bax, cleaved Caspase-3, and cleaved PARP. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Influence of FABP4 inhibitor on mouse high fatty liver transplantation model. A H&E staining of mouse liver transplantation model after using FABP4 inhibitor. B TUNEL staining. C – F Liver function detection of ALT, AST, LDH, and TB. G – I Oxidative stress injury assay of GSH, MDA, and SOD. J – N Relative expression of FABP4, Bax, cleaved Caspase-3, and cleaved PARP. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: Transplantation Assay, Staining, TUNEL Assay, Expressing

    Transcriptomic profiles revealing the influence of FABP4 inhibitor. A Violin diagram of expression patterns of all genes. B Principal component analysis of the duplicate samples. C Heatmap of the differentially expressed genes. D Volcano plot of the differentially expressed genes between HF LT and HF BMS groups. E Gene ontology pathway enrichment for differentially expressed proteins. The circle sizes represent the number of genes enriched in pathways, and the circle’s color means significance. F KEGG pathway enrichment for differentially expressed proteins

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Transcriptomic profiles revealing the influence of FABP4 inhibitor. A Violin diagram of expression patterns of all genes. B Principal component analysis of the duplicate samples. C Heatmap of the differentially expressed genes. D Volcano plot of the differentially expressed genes between HF LT and HF BMS groups. E Gene ontology pathway enrichment for differentially expressed proteins. The circle sizes represent the number of genes enriched in pathways, and the circle’s color means significance. F KEGG pathway enrichment for differentially expressed proteins

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: Expressing

    Metabolomic profiles revealing the influence of FABP4 inhibitor. A Total ion chromatogram of spectral overlap comparison, with response intensity and retention time overlapping. B Principal component analysis of the duplicate samples. C Volcano plot of the differentially expressed metabolites between HF LT and HF BMS groups. D Heatmap of the differentially expressed metabolites. E Correlation analysis between metabolites and visualized in the form of correlation heatmaps. F Revealing the co-regulatory relationships between various metabolites by chord diagrams

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Metabolomic profiles revealing the influence of FABP4 inhibitor. A Total ion chromatogram of spectral overlap comparison, with response intensity and retention time overlapping. B Principal component analysis of the duplicate samples. C Volcano plot of the differentially expressed metabolites between HF LT and HF BMS groups. D Heatmap of the differentially expressed metabolites. E Correlation analysis between metabolites and visualized in the form of correlation heatmaps. F Revealing the co-regulatory relationships between various metabolites by chord diagrams

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: Comparison

    Influence of FABP4 inhibitor on cAMP signaling pathway. A – E Relative expression of HHIP, ADRB2, RAC2, and PKA. F , G The content of Prostaglandin i2 and N-oleoylethanolamine validated by ELISA. H – K The mRNA expression of Hhip, Adrb2, Rac2, and Adcy7 of HF LT and HF BMS groups. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Influence of FABP4 inhibitor on cAMP signaling pathway. A – E Relative expression of HHIP, ADRB2, RAC2, and PKA. F , G The content of Prostaglandin i2 and N-oleoylethanolamine validated by ELISA. H – K The mRNA expression of Hhip, Adrb2, Rac2, and Adcy7 of HF LT and HF BMS groups. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay

    Influence of FABP4 inhibitor on high fatty liver mitochondrion. A – C Transmission electron microscopy image of mouse liver in each group. A The mitochondrial structure was clear, and no autophagosomes were presented in the HF SHAM group. B Autophagosomes and autolysosomes were found in the HF LT group with mitochondrial swelling. C The mitochondrial structure of the HF BMS group was clear, and damaged parts of mitochondria were lytic. D – F Multiplex immunofluorescence staining of rhodamine reagent for detecting mitochondrial membrane potential. Green fluorescence represents mitochondrial membranous potential, red fluorescence represents apoptotic hepatocytes, and blue fluorescence represents nuclear staining. G – I Relative expression of DRP1 and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. AP, autophagosomes; ASS, autolysosome; LD, lipid droplets; M, mitochondria; RER, rough endoplasmic reticulum

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Influence of FABP4 inhibitor on high fatty liver mitochondrion. A – C Transmission electron microscopy image of mouse liver in each group. A The mitochondrial structure was clear, and no autophagosomes were presented in the HF SHAM group. B Autophagosomes and autolysosomes were found in the HF LT group with mitochondrial swelling. C The mitochondrial structure of the HF BMS group was clear, and damaged parts of mitochondria were lytic. D – F Multiplex immunofluorescence staining of rhodamine reagent for detecting mitochondrial membrane potential. Green fluorescence represents mitochondrial membranous potential, red fluorescence represents apoptotic hepatocytes, and blue fluorescence represents nuclear staining. G – I Relative expression of DRP1 and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. AP, autophagosomes; ASS, autolysosome; LD, lipid droplets; M, mitochondria; RER, rough endoplasmic reticulum

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: Transmission Assay, Electron Microscopy, Multiplex Assay, Immunofluorescence, Staining, Membrane, Fluorescence, Expressing

    Influence of FABP4 siRNA on in vitro hypoxia / reoxygenation model. A – D Flow cytometry was performed to detect the hypoxic injury-induced apoptosis of AML12 cells. E – H Liver function detection of ALT, AST, LDH, and TB using the supernatant of each group. I – P Relative expression of FABP4, PKA, RAC2, HHIP, ADRB, DRP1, and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Journal: Cellular and Molecular Life Sciences

    Article Title: Integrated multi-omic analysis identifies fatty acid binding protein 4 as a biomarker and therapeutic target of ischemia–reperfusion injury in steatotic liver transplantation

    doi: 10.1007/s00018-023-05110-1

    Figure Lengend Snippet: Influence of FABP4 siRNA on in vitro hypoxia / reoxygenation model. A – D Flow cytometry was performed to detect the hypoxic injury-induced apoptosis of AML12 cells. E – H Liver function detection of ALT, AST, LDH, and TB using the supernatant of each group. I – P Relative expression of FABP4, PKA, RAC2, HHIP, ADRB, DRP1, and MFN-1. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001

    Article Snippet: The other two groups of mouse LT were performed after confirmation of the injury target screened by proteomic analysis: (v) normal control donor liver transplantation group treated with fatty acid binding protein 4 (FABP4) inhibitor (BMS-309403, MedChemExpress) group (NC BMS group), and (vi) high-fat donor liver transplantation treated with FABP4 inhibitor group (HF BMS group).

    Techniques: In Vitro, Flow Cytometry, Expressing

    Determination of adipocyte-specific markers and AGMO activity during 3T3-L1 adipocyte differentiation. A: Oil Red O staining of differentiated (day 11, upper panel) and undifferentiated (day 0, lower panel) 3T3-L1 cells. B: Gene expression of late adipocyte markers adiponectin ( Adipoq ), leptin ( Lep ), peroxisome proliferator-activated receptor gamma ( Pparg ), and fatty acid-binding protein 4 ( Fabp4 ) was analyzed by RT-qPCR using TaqMan technology. Open bars and open circles represent undifferentiated cells at day 0 prior to differentiation; gray bars, full circles correspond to mature adipocytes at day 11 after exposure of 3T3-L1 cells to the adipocyte differentiation medium 1 and 2 (n = 3). C: AGMO enzymatic activity in cell pellets of 3T3-L1 exposed to the basal medium at day 0 (open bars and open circles) and cells supplemented with the differentiation medium 1 and 2 at day 11 (gray bars and full circles) of adipogenesis (n = 3). D: Agmo gene expression of cells at day 0 (open bars and open circles) and day 11 (gray bars and full circles) of adipocyte differentiation. E: Time course of AGMO activity during differentiation of 3T3-L1 cells exposed to the standard hormonal differentiation cocktail (diff. med. control) or a hormonal induction medium devoid of either IBMX, RGZ, DEX, or IBMX/RGZ and DEX/RGZ in combination (n = 5). F: Lipid droplet quantification by Bodipy and Hoechst staining using the CellProfiler™ analysis software of 3T3-L1 cells at day 0 and day 11 incubated with the complete differentiation medium (control) or differentiation medium with omitted IBMX, RGZ, DEX, or IBMX/RGZ and DEX/RGZ (three areas per well; n = 4). Mean ± SEM except for the boxplots in F, which show the median ± interquartile range (IQR). Whiskers range from minimum to maximum. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.

    Journal: Journal of Lipid Research

    Article Title: Adaptations of the 3T3-L1 adipocyte lipidome to defective ether lipid catabolism upon Agmo knockdown

    doi: 10.1016/j.jlr.2022.100222

    Figure Lengend Snippet: Determination of adipocyte-specific markers and AGMO activity during 3T3-L1 adipocyte differentiation. A: Oil Red O staining of differentiated (day 11, upper panel) and undifferentiated (day 0, lower panel) 3T3-L1 cells. B: Gene expression of late adipocyte markers adiponectin ( Adipoq ), leptin ( Lep ), peroxisome proliferator-activated receptor gamma ( Pparg ), and fatty acid-binding protein 4 ( Fabp4 ) was analyzed by RT-qPCR using TaqMan technology. Open bars and open circles represent undifferentiated cells at day 0 prior to differentiation; gray bars, full circles correspond to mature adipocytes at day 11 after exposure of 3T3-L1 cells to the adipocyte differentiation medium 1 and 2 (n = 3). C: AGMO enzymatic activity in cell pellets of 3T3-L1 exposed to the basal medium at day 0 (open bars and open circles) and cells supplemented with the differentiation medium 1 and 2 at day 11 (gray bars and full circles) of adipogenesis (n = 3). D: Agmo gene expression of cells at day 0 (open bars and open circles) and day 11 (gray bars and full circles) of adipocyte differentiation. E: Time course of AGMO activity during differentiation of 3T3-L1 cells exposed to the standard hormonal differentiation cocktail (diff. med. control) or a hormonal induction medium devoid of either IBMX, RGZ, DEX, or IBMX/RGZ and DEX/RGZ in combination (n = 5). F: Lipid droplet quantification by Bodipy and Hoechst staining using the CellProfiler™ analysis software of 3T3-L1 cells at day 0 and day 11 incubated with the complete differentiation medium (control) or differentiation medium with omitted IBMX, RGZ, DEX, or IBMX/RGZ and DEX/RGZ (three areas per well; n = 4). Mean ± SEM except for the boxplots in F, which show the median ± interquartile range (IQR). Whiskers range from minimum to maximum. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.

    Article Snippet: Twenty micrograms of samples were separated on a NovexTM WedgeWellTM 4–20% Tris-Glycine Gel (Fisher Scientific, Invitrogen), blotted onto PVDF membrane (Bio-Rad Laboratories, Inc, Hercules, CA), blocked with 5% skim milk (Sigma), and stained with either mouse anti-fatty acid binding protein 4 (FABP4) (1:1,000 dilution; Santa Cruz, Heidelberg, Germany) or mouse anti-PPARγ (1:500 dilution; Santa Cruz).

    Techniques: Activity Assay, Staining, Gene Expression, Binding Assay, Quantitative RT-PCR, Control, Software, Incubation